DEPARTMENT OF BIOLOGICAL SCIENCES BIOL 207 MOLECULAR GENETICS AND HEREDITY *3 (3-0-3)
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1 DEPARTMENT OF BIOLOGICAL SCIENCES BIOL 207 MOLECULAR GENETICS AND HEREDITY *3 (3-0-3) The chromosomal and molecular basis for the transmission and function of genes. The construction of genetic and physical maps of genes and genomes. Strategies for the isolation of specific genes. Examples of regulatory mechanisms for the expression of the genetic material in both prokaryotes and eukaryotes. Prerequisite: Biology 107. Term: September - December 2002 Place/Time: ETL E1 007/ MWF, 12:00-12:50 Lecturer: Dr. Heather McDermid Course Coordinator : Dr. John Locke Lecturer: Dr. Heather McDermid Office: Room G508A, Bio.Sci.Bldg. Ph Office Hours: open door (see schedule on WWW) hmcdermi@ualberta.ca Required textbook "An Introduction to Genetic Analysis - 7 th Edition" (2000) by Griffiths et al. This is available at the U. of A. Bookstore. The "Student Companion" book has answers to the problems listed at the back of each chapter in the textbook. The older texts, Griffiths 6 th Edition and Fairbanks & Andersen, are no longer recommended. I will assume you have the Griffith's text (7th Edition) and will provide page references for this text. Some background material can also be found in the Biol207 Laboratory Manual. Supplementary information/readings may also be handed-out, or assigned in class. Weekly Laboratories There is a three-hour laboratory session each week. Attendance at laboratory sessions is required. Section: A2 The laboratory coordinator is: Dr. Ramesh Bhambhani (Rm. CW312, Bio.Sci.Bldg ). The course technician preparing the labs is: Ms. Stacey Mebs (Rm G104 Bio. Sci. Bdg ). The first laboratory session contains significant work and will take place in the second week of term (Sept ). If you have not yet registered for a laboratory section, you must contact Dr. Bhambhani as soon as possible. The Laboratory Manual is on sale in the U of A Bookstore. Purchase your copy as soon as possible and well before your first lab period. You must read Exercise 1 before arriving in the lab. There is a worksheet to do in the first Lab period. The Final Lab Exam is scheduled for: 10:00 AM 1:00 PM Saturday, Nov 29, Notice about Cheating and Plagiarism Cheating and Plagiarism are serious academic offences and are not permitted. See the Code of Student Behavior (Section 26 in the student Calendar) and the web site, for the latest revision. Students caught cheating or plagiarizing will be dealt with according to the current Code of Student Behavior. Biol207 Syllabus: Sept-Dec. 2003, Section A2, Dr. McDermid, 12noon, MWF, ETL E1 007 Page 1
2 Lecture content Material for the lecture portion of this course comes from four main sources: lectures (and related web notes), text readings, problems & questions assigned in the text, and supplementary readings. (There is also significant overlap with the lab content so as to reinforce certain key concepts.) Students who take advantage of all sources (including the workshops - see below) to their fullest extent will be more likely to do better on the midterm and final exams. Grade Assignment and Exams The final grade will be based on the following distribution: 40% Laboratory Marks (Lab reports, quizzes, exam) 20% Mid-term Exam (in lecture period #20) 40% Final Exam The 40% of the course grade attributed to your performance in the Laboratory segment of the course will be explained in more detail in your Laboratory. There are 10 laboratory exercises for which Lab Reports or Quizzes may be required. There is a Lab Exam scheduled for 10:00 AM Saturday Nov 29 '03 There will be a Mid-term Exam during the lecture period #20 (Mon,Oct 20). Students who obtain an "Excused Absence" from the Mid-term Examination will have the 20% weight transferred to their Final Examination performance. The Final Exam for Section A2 is currently scheduled by the University to be on: Wednesday, December 10 at 2:00-5:00 PM Absent from an exam? Anyone with a valid excuse for missing an exam date should see sections 23.5 in the 2003/2004 UofA Calendar (starts on p 65). The Deferred Final Exam for Biol207 Section A2 will be held on Monday Jan. 5, 2004 at 09:00 AM. Meet in room G508 Bio. Sci. Building. This is the only date and time for the deferred final exam for Biol207 Section A2. NEW: FOUR-POINT LETTER GRADING SYSTEM September 2003 marks the introduction of the new four-point letter grading system : see the following U. of A. web site for more details l#6 The final grades for Biol 207 will be an A+, A, A-, B+, B, B-, C+, C, C-, D+, D, or F according to the guidelines in the above web site. The 1 through 9 system will not be used. Also see World Wide Web Pages for Biol207 WWW pages have been constructed for the Netscape and Internet Explorer web browsers (most other web browsers will also work). T h e a d d r e s s It is also accessible through the Department of Biological Sciences Home Page ( ). These Biol207 pages provide more detailed course information, readings, and lecture notes. The "notes" are primarily an on-line version of the overheads presented in class. Students may download the text to make inclass note-taking easier. These notes are not a substitute for the course or for attendance at lectures. They are not complete lecture notes. "Current news" is also available, such as changes in class content or corrections to the web pages. Your comments and contributions are also welcome. The Laboratory Manual is not available on the net. You must buy the Laboratory Manual (see above) from the Bookstore. Biol207 Syllabus: Sept-Dec. 2003, Section A2, Dr. McDermid, 12noon, MWF, ETL E1 007 Page 2
3 Biol 207-A2 LECTURE OUTLINE September - December, 2003 The dates, lecture numbers and titles are listed. Suggested readings and problems assigned will be available on the McDermid 207 website as the webnotes for each lecture are made available. Date Lecture # and Title Wed Sep 3 1. Course Intro + DNA is the genetic material Fri Sep 5 2. Chromosome replication 1 Mon Sep 8 3. Chromosome replication 2 Wed Sep Genes encode proteins 1 Fri Sep Genes encode proteins 2 Mon Sep Mutations originate as damage to DNA Wed Sep DNA repair Fri Sep Classification of mutations Mon Sep Introduction to gene regulation and operons Wed Sep Induction of the lac operon Fri Sep Eukaryote gene structure and regulation Mon Sep Alleles, Dominance & Segregation (Mendel's 1 st law) Wed Oct Chromosomes Fri Oct Chromosome behavior in Meiosis, and life cycles Mon Oct Genetic implications of Meiosis Wed Oct Independent assortment, dihybrid crosses Fri Oct Linkage Mon Oct 13 Thanksgiving Monday - no class Wed Oct Sex chromosomes and sex linkage Fri Oct Catch-up Mon Oct * * * * Midterm exam * * * * Wed Oct Mapping 3 loci (3 point crosses) 1 Fri Oct Mapping 3 loci (3 point crosses) 2 Mon Oct Pedigree analysis Wed Oct Chromosome rearrangements Fri Oct Changes in chromosome number - Ploidy Mon Nov Gene interactions and epistasis Wed Nov Physical mapping of genes, chromosomes Fri Nov Genome projects Mon Nov 10 Remembrance Day Holiday - no class Wed Nov Clones and DNA libraries Fri Nov Identifying genes by complementation Mon Nov Identifying clones using DNA probes Wed Nov Isolation of genes by Transposon Tagging Fri Nov Gene manipulation Mon Nov PCR and it s uses Wed Nov Restriction Fragment Length Polymorphism (RFLP) 1 Fri Nov Restriction Fragment Length Polymorphism (RFLP) 2 Mon Dec Mendelian and molecular genetics combined Wed Dec catch-up Copyright 2002 Heather McDermid, Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada Biol207 Syllabus: Sept-Dec. 2003, Section A2, Dr. McDermid, 12noon, MWF, ETL E1 007 Page 3
4 Notes about assigned problems for lectures: Most lectures have assigned problems. The only way to learn the concepts in this course is to do the problems. Answers to all these problems can be found in the "Solutions Manual" (soft cover) that came with the text (hard cover). Look at the answer ONLY after you have tried your best at the problem. Just reading the answer will not help you learn the concepts. Don t understand the problem? - look again and review the text material. Still don t understand the problem? See Dr. McDermid or the tutorial assistant. More problems: Sample midterm and final exams will be available at Dr. McDermid's Biol207 Web site. More problems will also be presented in the weekly workshops by the TAs. Biol 207 Workshops: The Department of Biological Sciences has received funding from the VP Academic to add new "Workshops" to the first-year and second-year core courses. These smaller-group classes come in response to student s concerns over the problems inherent in large introductory classes. The objectives of the Biol207 Workshops are: 1. To increase the quantity and quality of interaction between undergraduate students and instructional staff of the University so that students may learn better. 2. To promote "active learning" and enhanced understanding of the Biology 207 curriculum through student participation in topic reviews and problem solving. 3. To provide students with timely feedback on their learning of the Biology 207 curriculum so that they may correct any deficiencies in Biol207 content or understanding prior to examination. 4. To improve student performance on Biol207 exams. Workshop Registration: Every student should be registered in a Biology 207 Workshops; it is mandatory. There are 10 weeks of sessions and the first begins week of September 15 th. (See schedule below.) Each Workshop is a 50-minute session. All Workshops will take place in G116 Biological Sciences Building (basement of the Genetics wing - south west side) at the following days and times: Section A1 (Dr. Locke) Section A2 (Dr. McDermid) E01 Monday 9:00-9:50AM E02 Monday 10:00-10:50AM E03 Thursday 2:00-2:50PM E06 Tuesday 9:00-9:50AM E07 Tuesday 10:00-10:50AM E08 Tuesday 11:00-11:50AM E04 Thursday 3:00-3:50PM E05 Thursday 4:00-4:50PM E09 E10 Wednesday 4:00-4:50PM Wednesday 5:00-5:50PM Biol207 Syllabus: Sept-Dec. 2003, Section A2, Dr. McDermid, 12noon, MWF, ETL E1 007 Page 4
5 Biol207 Workshop Schedule - Fall Sept-Dec Date Workshop# Lecture# Sept 3-5 No workshops 1-2 Sept 8-12 No workshops 3-5 Sept Workshop#1-6-8 Sept Workshop# Sept 29-Oct 3 Workshop# Oct 6-10 Workshop# Oct Workshop#5 - Midterm exam review Oct Workshop# Oct Workshop# Nov 3-7 Workshop# Nov No workshops Nov Workshop# Nov Workshop#10 - Final Exam Review Dec 1-3 No workshops Note: The best way to study for Biol207 exams is to "Do the problems!" Of the students last year (Fall term Sept-Dec'02) who received a grade of either 8 or 9, onethird went to 8-10 of the workshops and two-thirds went to five or more workshops. Use these workshops to improve your understanding and your grade. Biol207 Syllabus: Sept-Dec. 2003, Section A2, Dr. McDermid, 12noon, MWF, ETL E1 007 Page 5
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