General Microbiology

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Course Overview (BIOL 142, 4 cr.) A survey of microbial cell biology, microbial genetics, the impact of microorganisms on the environment, and microbial interactions with humans; emphasizing human infectious disease and including a survey of immunology. Instructor: Paul Lepp, Ph.D. Office: Moore 221 Phone: 858-3508 Email: paul.lepp@minotstateu.edu Office hours by appointment. Course Material There is no required printed text for the lecture portion of this course. There is a required lab manual which may be purchased at the campus bookstore. The course will make use of various online resources and handouts. The primary text for this course will be: Microbiology By Parker, Schneegurt, Tu, Forster and Lister https://openstax.org/details/books/microbiology Course website: http://yourspace.minotstateu.edu/paul.lepp/microbiology/generalmicrobiology.html The Laboratory Manual is required for the course and may be purchased at the campus bookstore. Course Information and Prerequisites (BIOL 142) meets for lecture every Monday, Wednesday and Friday from 12:00 to 12:50 pm in 114 Memorial Hall. All labs will meet in 318 Moore Hall. Lab meeting times are Tuesday at 10 am and 1 pm and Thursday 1 pm. Learning Objectives Both the lecture and laboratory components of at Minot State University (BIOL 142) are designed to adhere to the 5 core themes and concepts recommended for an Introductory Mi-

crobiology course by the American Society for Microbiology. At the conclusion of the course the student will have mastered the following skills: Proper use of the microscope, Gram staining, streak isolation, the ability to carry out a dilution series and the ability to interpret various biochemical tests in order to identify bacterial species. Lecture Lecture attendance is at your discretion. We will cover subject matter not in the online textbook and I believe lecture will also serve as a useful guide to a very diverse subject. In addition, the date of chapter pop quizzes will be announced in class. Lecture will also provide a forum for you to ask questions and review problems. NO CELL PHONES! Turn them off or put them on vibrate before you come to class. Study To succeed in this class I expect that you will have to spend at least 2 hours on the course material outside of class for every hour of lecture. You will be responsible for the material from lecture, the book and supplemental readings. I strongly encourage you rewrite your notes in a timely fashion. Study Guides will be posted online several days before each lecture quiz. Assessment and Grading Your final grade will be based upon lecture exams, a comprehensive final exam, pop quizzes, and lab grade. There are 4 exams given throughout the term. Each quiz will consist of multiple choice questions. You will not be quizzed on material we have not covered. However, you are responsible for all assigned material. There will be no make up exams. I also reserve the right to administer pop quizzes, which will be factored into your grade. The quiz and exam dates are given in the schedule below. Please email or see me by the end of the second week of classes to schedule an alternative date for quizzes if you have a conflict due to a MSU sanctioned event. This will be the only opportunity to schedule alternative quiz dates. My office phone and email are listed at the top of the syllabus, as well as, in the campus directory and with the main switchboard. There is little excuse not to contact me in the event that you cannot attend a quiz. As a matter of policy I do not issue extra credit. See below for details on laboratory grading. Tentative Course points 4 lecture quizzes (50 pts each) 200 6 reading quizzes (drop 1; 5 pts each) 25 final exam (can not be dropped) 100 12 lab reports (drop 1; 10 pts each) 120 2 lab practical exams (25 pts each; can not be dropped) 50 total: 495 It is anticipated that the grading scale will follow a strict 90% (A), 80% (B), 70% (C), 60% (D) scale.

It is the responsibility of the student to retain all graded material and present it should there be a discrepancy in grades. Laboratory Each laboratory exercise is accompanied by a lab report. Lab reports are due on the day all of the necessary data for the lab exercise has been collected. Each laboratory report will be worth 10 points. Late assignments will not be accepted. Due to limited resources labs may not be made-up. You will be allowed to drop one lab report grade. If you miss a lab the missed lab becomes your dropped lab report grade. There are also two lab practical exams which may not be made-up or dropped. The lab practicals will be graded on a curved scale to discourage students from communicating the content of the exams to students in other lab sections. The biohazard buckets in lab may not be used for non-hazardous material. Any student placing non-hazardous material in a biohazard bucket will receive a zero for that week s lab report. Ms. Lori Ihli will be teaching the Tuesday afternoon lab section. You can reach her through campus email: lori.ihli@minotstateu.edu Academic Honesty (per undergraduate catalog) Honesty and integrity are central to academic life at Minot State University. They create a trust necessary in a community of scholars. When that trust is violated by cheating in any form, the atmosphere of academic freedom is threatened. Cheating may affect the student in accordance with the faculty member s grading policy, and/or it may result in student disciplinary action in accordance with the Student Conduct Policy (available in the office of Student Affairs). Privacy You will be asked write your class assigned ID number on exams and homework. Graded exams and assignments will be available outside my office door. Writing your name on exams and homework will be taken as an implicit acceptance that your grade may be revealed to other students. Students with disabilities In coordination with Disability Support services, reasonable accommodations will be provided for qualified students with disabilities (LD, orthopedic, hearing, visual, speck,, psychological, ADD/ ADHD, health related or other). Please contact the instructor during the first week of class to make arrangements. Additional information is available from Disability Support Services at 858-3371 for evelyn.klimpel@minotstateu.edu. Minot State University interpersonal abuse statement (Title IX)

Minot State University strives to create a campus community free from interpersonal abuse including sexual misconduct. In working to achieve this intent, Minot State University commits to: Taking action to stop sexual misconduct; Taking action to remedy its effects by providing advocacy, support and appropriate referral services for recipients of the behavior; Taking action to prevent recurrences; Educating individuals and promoting discussions on interpersonal abuse and violence; and conducting impartial investigation of all reports/notices of sexual misconduct through fair, equitable and prompt procedures. Investigations will be independent of and separate from law enforcement investigations of criminal activity. People to contact if you have either experienced or have seem someone experiencing interpersonal abuse: Lisa Dooley (MEM 401, 858-3447); Steve Swenson (Dome, 236C, 858-3040); Wes Matthews, ADM 2 nd Floor, 858-4610); Kevin Harmon (ADM 1 st floor, 858-3299); or Gary Orluck (Plant Services building, 858-4016). You can also contact these persons in writing. Holidays, Vacations January 15 Martin Luther King Day February 19 President s Day March 12-16 Spring Break March 30-April 2 Good Friday & Easter Break April 14 No Class Quiz Dates February 2 March 2 March 29 April 27 May 8 - Final @noon, 114 Memorial Hall Lecture Schedule Week 1 Microbiology & the origin of life - Ch. 1 Week 2 Origin of Life & Bacterial Diversity; Cell Structure - Ch. 3 & 4 Week 3 Cell structure cont. Week 4 Chemistry of life - Ch. 7 Week 5 Physiology & Genetics - Ch. 8, 10 & 11 Week 6 Physiology & Genetics Week 7 Physiology & Genetics Week 8 Microbial Growth - Ch. 9 Week 9 Control of Microbial Growth & Antimicrobial - Ch. 13 & 14 Week 10 Antimicrobials & Normal flora Week 11 Pathogenesis - Ch. 15 Week 12 Pathogenic microbiology Week 13 Pathogenic microbiology Week 14 Virology - Ch. 6 Week 15 Virology Week 16 Microbial Ecology

Laboratory Schedule Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7 Week 8 Week 9 Week 10 Week 11 Week 12 Week 13 Week 14 Week 15 Week 16 No Lab Microscopy Aseptic Technique & Cultivation; Winogradsky Columns Staining Normal Flora & Antibiotic Resistance Epidemiology & Antibiotic Resistance Handwashing, Antibiotic Resistance Lab Practical Quiz, Antibiotic Resistance & Handwashing results Microbial Growth & Antibiotic Resistance Control of Microbial Growth Yogurt production; Winogradsky Columns Kirby Bauer Assay Kirby Bauer Assay; Identification of an Unknown Identification of an Unknown Identification of an Unknown Lab Practical Quiz