APPLIED FISHERIES STATISTICS

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APPLIED FISHERIES STATISTICS Overview: Fall 2016 FAS 5335C (section 143H) and FAS 4932 (section 143E) 4 credit hours Tuesdays and Thursdays: Periods 3-4 (9:35am 11:30am) MAEB Room 234 Ever wonder what to do with all that data? Too much data? Not enough data? Right kind of data? Maybe you should have thought of that before you ever collected it! The goal of this course is to help you organize your data (past or future) and to learn how to apply many of the statistical tests (that you have learned, should have learned, or will learn) to data collected from aquatic systems, along with learning some new methods of sampling, analysis, and presentation. Topics will include mathematical distributions, transforming data, outliers, significant figures, number of samples needed, effect of sampler size, sample design, mark-recapture and depletion methods of estimating abundance, lengthfrequency analysis, length-weight relationships (K, W r, ANOCOVA), and basic statistical tests (e.g., t-tests, paired t-tests, tests of normality, correlations, simple ANOVAs, regression analysis). Additional topics will include ratios, pseudoreplication, nonparametric statistics, repeated-measures ANOVA, multiple comparison testing, and variable selection techniques. Handouts (computer printouts and primary literature) will be used extensively as supporting materials. Students will learn the basics of SAS (Statistical Analysis System), JMP and EXCEL programming for data management and analysis, along with being introduced to R. Grades will be based on weekly/biweekly problems sets and a class project. Each student will conduct an independent "sampling" project on a fisheries or aquatic science topic of their choice, including review of the literature, proposal and budget preparation, completion of field and/or laboratory work, and preparation of paper and oral presentation based on their research.

Instructor: Dr. Chuck Cichra Professor / Extension Fisheries Specialist University of Florida / IFAS SFRC - Fisheries & Aquatic Sciences 7922 NW 71st Street (Room 27, Bldg. 544) Gainesville, Florida 32653-3071 Office: (352) 273-3621 Cell: (352) 339-6173 Fax: (352) 392-3672 Email: CECichra@ufl.edu Website: http://sfrc.ufl.edu/people/faculty/cichra/ Teaching Assistant: Mrs. Crystal Hartman Biological Scientist University of Florida / IFAS SFRC - Fisheries & Aquatic Sciences 7922 NW 71st Street (Room 25 and 26, Bldg. 544) Gainesville, Florida 32653-3071 Office: (352) 273-3622 Cell: (352) 214-8179 Fax: (352) 392-3672 Email: c.hartman@ufl.edu Office Hours: Call or e-mail for an appointment, meet after class, or stop in if our doors are open. Feel free to call or e-mail questions to either of us. Course Website: This course will be supported by a UF e-learning CANVAS website located at https://lss.at.ufl.edu/. It will include the course syllabus, PowerPoint presentations, recommended readings, handouts, course assignments, proposal format and budget Excel files, presentation and paper guidelines, and other materials. 2

Directions: From UF Campus 1) From campus at the corner of W. University Ave. and NW 13th St. (US 441) go north to NW 53rd Ave. (3.5 mile). Go west past NW 43rd St. and the Devil's Millhopper State Geologic Site to NW 71st St. (4.6 mile). Turn right (north) and go to the end of the paved road (1.1 mile). Fisheries and Aquatic Sciences and the Center for Aquatic and Invasive Plants buildings are on the right at end of paved road. The Administration Building is the brick building to the east (your right). Our offices are in the longest steel strand building. 2) Alternatively, from W. University Ave., you can take NW 34th St. north to NW 39th Ave. (2.5 mile). Turn left (west) and go to NW 43rd St. (1 mile). Turn right. Drive north on NW 43rd St. to NW 53rd Ave. (1 mile). Turn left (west) and proceed as above. From Interstate 75 Take Exit 390 at NW 39th Ave. Drive east to NW 43rd St. and proceed as above. There is no exit at NW 53rd Ave. 3

Grading: Problem Sets Class Project Points 600 Take-home (~8 problem sets) 15 Project pre-proposal 110 Project proposal 175 Project paper 100 Final oral presentation Total: 1000 Grade Points Grading Scale A 931-1000 A- 900-930 B+ 871-899 B 831-870 B- 800-830 C+ 771-799 C 731-770 C- 700-730 D+ 671-699 D 631-670 D- 600-630 E 0-599 Problem sets must be turned in to Dr. Cichra by the beginning of lecture on the assigned due date. Project-related work must be turned in to Dr. Cichra or to the Fisheries and Aquatic Sciences' main office by 5:00 PM on the assigned due date. 10% of the assignment's total value will be deducted per day for any work not turned in on time. Excused tardiness for course work will be granted if an acceptable excuse if provided. If you know that you will not be in town on due dates, pleases make prior arrangements for turning in assignments (preferably early if possible). Assignments can be faxed or E-mailed. 4

APPLIED FISHERIES STATISTICS - 2016 Schedule DRAFT (This schedule will change by Sep 04 with input from students, and from other instructors. Tentative due dates for problem sets will be added) Tuesday Thursday Aug 23 Introduction to course Aug 25 Aug 30 Sep 01 Sep 06 Sep 08 Sep 13 3086 McCarty B - SAS Lab Sep 15 Pre-proposal due Sep 20 Sep 22 No Class! Sep 27 Sep 29 EXCEL Workshop Oct 04 3086 McCarty B Lit. Lab Oct 06 Proposal due??? Oct 11 Oct 13 Oct 18 EXCEL II Workshop Oct 20 Oct 25 Oct 27 Nov 01 User Surveys / Qualtrics Nov 03 SAS JMP Workshop Nov 08 Nov 10 Nov 15 Nov 17 Nov 22 Nov 24 No Class - Thanksgiving Nov 29 Papers due Dec 01 Dec 06 NO FINAL Exam Oral Presentations Classes will be held every Tuesday and Thursday, unless announced otherwise. Classes will consist of primarily lectures/discussions, along with computer sessions (SAS, JMP, and EXCEL). 5

Course Topics / Outline: Organize / Explain Course Purpose, instructors, topics, grading, schedule, project, proposal, paper, presentation Common sampling techniques Sampling and basic statistics Mean, median, mode, standard deviation, variance Significant figures Sampling designs Distributions Terminology Histograms Value in sampling Mathematical frequency distributions - generation and application Positive binomial Poisson series Negative binomial Normal Normality Tests for normality Violation of statistical assumptions What do you do if your data are not normal? Transformations What are transformations? Why do we transform data? Methodologies Common transformations for fisheries and aquatic science data Tests to see if transformations worked If you can't transform, then what? Non-parametric statistics? Outliers What are they? Methods to see if you have them (Detection) Effects of outliers on analyses What do you do with them when detected? Catch-per-unit-of-effort (CPUE) sampling Number of samples Size of samples(r) Mark-recapture techniques What is it? Why use it? Assumptions What happens if you violate them? How much can you violate them? 6

Methodology Biases Calculating confidence limits Sample sizes - allocation of effort (M vs C) Estimating fish abundance by depletion Zippin method Comparing distributions Size distributions Age structure Chi-square analysis Kolmogorov-Smirnov test Length-weight relationships Condition factors (K) Relative weights (Wr) Methodology Standard weights Length-weight regressions Analysis of covariance Length-frequency analysis Histograms Anderson's numerical analyses Proportional stock density (PSD) Relative stock density (RSD) Empirical modeling What is it? Why use it? An example using regression analysis Pseudo-replication Repeated-Measures Analysis of Variance Multiple comparison testing Statistical methods (SAS examples will be used throughout the course) T-test Paired t-test Analysis of variance (1-way, 2-way, interaction, etc.) Analysis of covariance Correlation analysis Regression analysis Simple / Multiple Variable selection techniques (forward, backward, stepwise) Non-parametric 7

FAS 5335C / FAS4932 - Applied Fisheries Statistics Project Paper Grading Sheet Content POINTS Abstract 9 Goal of study, objectives, introduction 17 Description of study site & methods 35 Analysis of project data (including project design) 35 Literature review 17 Overall discussion 26 Style Readability 7 Follows AFS guidelines 18 Neatness, grammar 11 Total 175 COMMENTS: 8

Pertinent References: Boyd, C.E. 1979. Water quality in warmwater fish ponds. Auburn University, Auburn Experiment Station, Auburn, Alabama. 359pp. Carlander, K.D. 1950. Handbook of Freshwater Fishery Biology, Volume One. The Iowa State University Press, Ames, Iowa. 752pp. (Freshwater fishes exclusive of the Perciformes) Carlander, K.D. 1977. Handbook of Freshwater Fishery Biology, Volume Two. The Iowa State University Press, Ames, Iowa. 431pp. (Centrarchids) Elliott, J.M. 1971. Some methods for the statistical analysis of samples of benthic invertebrates. Freshwater Biological Association, Scientific Publication No. 25. 148pp. Guy, C.S. and M.L. Brown. 2007. Analysis and interpretation of freshwater fisheries data. American Fisheries Society, Bethesda, Maryland. 961pp. Hoyer, M.V. and D.E. Canfield, Jr. 1994. Handbook of common freshwater fish in Florida lakes. SP160. University of Florida, Gainesville, Florida. 189pp. Kohler, C.C. and W.A. Hubert. (Editors) 1993. Inland fisheries management in North America. American Fisheries Society, Bethesda, Maryland. 594pp. Murphy, B.R. and D.W. Willis. (Editors) 1996. Fisheries Techniques, Second Edition. American Fisheries Society, Bethesda, Maryland. 732pp. Novinger, G.D. and J.G. Dillard. (Editors) 1978. New approaches to the management of small impoundments. Special Publication No. 5. American Fisheries Society, Bethesda, Maryland. 132pp. Ricker, W.E. 1968. Methods for assessment of fish production in freshwaters. IBP Handbook No. 3. Blackwell Scientific Publications, Oxford, England. 313pp. Ricker, W.E. 1975. Computation and interpretation of biological statistics of fish populations. Bulletin 191. Fisheries Research Board of Canada, Ottawa, Canada. 382pp. Seaman, W. (Editor) 1985. Florida aquatic habitat and fishery resources. Florida Chapter, American Fisheries Society, Eustis, Florida. 543pp. Summerfelt, R.C. and G.E. Hall. (Editors) 1987. Age and growth of fish. The Iowa State University Press, Ames, Iowa. 544pp. Numerous pieces of primary literature, sample data analyses, computer programs, manuals, and reports will be handed out and/or placed onto the course CANVAS e- learning website during this semester. 9

Academic Honesty: As a result of completing the registration form at the University of Florida, every student has signed the following statement: I understand that the University of Florida expects it students to be honest in all their academic work. I agree to adhere to this commitment to academic honesty and understand that my failure to comply with this commitment may result in disciplinary action up to and including expulsion from the University. The Honor Code ( http://www.dso.ufl.edu/sccr/process/student-conduct-honor-code/ ) specifies a number of behaviors that are in violation of this code and the possible sanctions. Furthermore, you are obligated to report any condition that facilitates academic misconduct to appropriate personnel. If you have any questions or concerns, please consult with the instructor or TAs in this class. UF Counseling Services: Resources are available on-campus for students having personal problems or lacking clear career and academic goals which interfere with their academic performance. These resources include: 1. University Counseling Center, 301 Peabody Hall, 392-1575, personal and career counseling - http://www.counseling.ufl.edu/cwc/default.aspx 2. Student Mental Health, Student Health Care Center, 392-1171, personal counseling; 3. Sexual Assault Recovery Services (SARS), Student Health Care Center, 392-1161, sexual assault counseling; and 4. Career Resource Center, Reitz Union, 392-1601, career development assistance and counseling. Software Use: All faculty, staff and students of the University are required and expected to obey the laws and legal agreements governing software use. Failure to do so can lead to monetary damages and/or criminal penalties for the individual violator. Because such violations are also against University policies and rules, disciplinary action will be taken as appropriate. 10