BIOL&260 Microbiology Winter 2019 Meeting Times: Lecture Monday and Wednesday 5:30 6:45 PM LSC-111 Laboratory Monday and Wednesday 6:50 8:40 PM LSC-147 Course Requirements: This is a 200 level transfer course and requires an understanding of basic biological and chemical principles as well as good study skills. Both Biology &160 and Chemistry 100 are prerequisites for this course. Instructor: Dr. Becky Olsen-Grady E-mail: please email me thru Canvas or at rolsengrady@pierce.ctc.edu Office Hours: Office hours are by appointment, please email me to schedule a time TEXTBOOK required Lecture: Microbiology, Tortora, Funke, Case; 11 th edition Laboratory: Microbiology: Laboratory Theory and Application, Leboffe and Pierce COURSE OVERVIEW Biology&260: Microbiology is a biological science class that presents an overview of the study of organisms not visible to the naked eye. It includes the study of bacteria, fungi, algae, protozoa, viruses and prions and their vital roles on this living planet. We will also study their involvement in several infectious diseases. COURSE OBJECTIVES Upon successful completion of this course, the student will be able to: 1) Understand basic principles of microbiology including cell structure, biochemical pathways, genetics, replication and evolution of cells 2) Understand the role of microbes in the environment and in causing diseases 3) Master basic microbial laboratory procedures 4) Develop the ability to collect, record and analyze data 5) Develop ability to read, interpret and critique articles about microbiology and to communicate that information to an audience 6) Increase your ability to work constructively with others 7) Expand your awareness and appreciation of the invisible organisms all around us
Strategies for Success in this Class: Attend all lecture periods and laboratories. Take notes, review (re-write) them and use them as a study guide. Stay current with the class (readings, notes, laboratory exercises, etc.). Focus on understanding the Big Picture and then add in the details Study by yourself and also with fellow students.. ASK QUESTIONS, ask questions, ask questions... of yourself when you are studying, your fellow students, and myself. I am happy to answer questions during lectures, labs, office hours or by e-mail. INSTRUCTOR RESPONSIBILITIES To facilitate learning of course objectives. To provide a comfortable classroom environment in which student success is attainable. To facilitate constructive and appropriate group and class discussion of topics. To make supplemental material available for students through Canvas. To be available to answer questions by appointment and via e-mail at least once a day during the week. STUDENT RESPONSIBILITIES To be on time to lecture and lab. If absence is unavoidable, it is your responsibility to notify the instructor and obtain notes from fellow students. To keep track of due dates as presented in the syllabus or by instructor. To keep up with readings and turn in assignments on time. To study outside of class hours. Do not be afraid to ask questions. If you have a question, you are probably not alone. If you are having trouble understanding the material or doing well on quizzes or exams, it is your responsibility to seek help from me outside of class. To provide a comfortable learning environment for your fellow students. You must treat me and your fellow classmates with respect. No discriminatory comments or attitudes towards your fellow classmates for any reason will be tolerated, including but not limited to race, ethnicity, sexual orientation, gender, or disability. To interact with your classmates during class and lab. This means that you must be an engaged student and must participate in group activities. You will not succeed in this course by sitting back and letting your group members do all the work. POINTS BREAKDOWN Grades in this class will be determined based on points received in the following categories LECTURE Exams 4 at 100 pts each Chapter Quizzes 15 at 10 pts each Lowest will be dropped Homework (Lecture or Lab related) 400 pts 130 pts 50 pts
LABORATORY Laboratory Quizzes Lowest will be dropped Laboratory Assignments Laboratory Exams Unknown Project Total points 100 pts 150 pts 60 pts 60 pts 950 pts The total number of points will be used to determine your percentage. You can use the table below to convert percentage points to your grade. I DO NOT ROUND OR CURVE GRADES. The earned percentage is then apportioned as follows: 4.0 = 95%+ 3.4 = 89% 2.8 = 83% 2.2 = 77% 1.6 = 71% 1.0 = 65% 3.9 = 94% 3.3 = 88% 2.7 = 82% 2.1 = 76% 1.5 = 70% 0.0 < 65% 3.8 = 93% 3.2 = 87% 2.6 = 81% 2.0 = 75% 1.4 = 69% 3.7 = 92% 3.1 = 86% 2.5 = 80% 1.9 = 74% 1.3 = 68% 3.6 = 91% 3.0 = 85% 2.4 = 79% 1.8 = 73% 1.2 = 67% 3.5 = 90% 2.9 = 84% 2.3 = 78% 1.7 = 72% 1.1 = 66% LATE ASSIGNMENTS Late assignments will only be accepted in circumstances where you can provide documentation of the reason for your absence. GENERAL INFORMATION Exams: Exams will be a combination of multiple choice, true/false, matching, essay and critical thinking questions. You will be assessed on recall of terminology, processes, and structures and asked to explain details of how and why things work. Each exam will focus on the material covered in lecture prior to the exam itself. The final exam is not cumulative. LABORATORY: In a microbiology class, laboratory is a very important component of the class. During each laboratory period you will take notes, make drawings of your observations and answer questions about that exercise. BEFORE coming to lab you should read and understand the
theory, application and in this exercise portion of each laboratory exercise as well as the appropriate section in your textbook. You should also scan the materials and procedures portion so that you are well prepared to do the exercise. During the last half of the quarter, you will be given a culture with a microorganism that you will need to isolate and identify. You will be graded on your success in this task and on your laboratory notebook and flow chart (60 pts.) You will be given more information about this project later. Laboratory Quizzes and Projects There will be laboratory quizzes at the beginning of each lab period to ensure that you have read and prepared for the days lab. There will also be projects related to lab that will be announced later. Laboratory Safety: For safety reasons, children, food, and drink are not permitted in the laboratory and children are not allowed to attend lecture. Copied assignments and plagiarism: It is my firm belief that unless you can express something in your own words, you do not truly understand it. As all assignments are designed to build and assess your understanding, all assignments must be written in your own words. Wording that is copied from the text, the lab manual, Bergey s Manual, internet sources or other students will receive no credit. Copying on exams will result in a zero grade for the exam and disciplinary action according to the Student Handbook. Tape recording: You are welcome to tape my lectures for the sole purpose of assisting yourself with learning the material. Cell phones: All cell phones should be turned off during class and during laboratory. If you must text do so discreetly and quickly only in emergencies and/or step into the hallway to do so. Course withdrawal: If you decide to drop this course for any reason, you must officially withdraw with the registration office or you will receive a failing grade in the course. I do not offer any of the exception grades like instructor withdrawal or audit.
Laboratory Schedule Fall 2018 (May be subject to change) Dates Monday Wednesday 1/7+1/9 Introduction to Lab Safety Aseptic Transfers Investigate Prepared Microscope Slides Streak Plate Receive Stock Cultures Simple Staining Negative Staining 1/14+1/16 Colony Morphology and Transfer to slant Gram Stain Endospore Stain Acid-Fast Stain Read, not performed 1/21+1/23 NO LAB MARTIN LUTHER KING JR. DAY Growth Patterns on Slants: Observe your transfers Phenol Red Methyl Red and VP PEA, Mannitol Salt MacConkey, EMB Blood Agar 1/28+1/30 Anaerobic Jar Oxidation-Fermentation Catalase Slant NOT slide test Practice Gram Stain for Wednesday Gram stain portion of the practical Table by table will smear and perform Gram stain without your lab text Effects of Temperature Effects of ph 2/4+2/6 LAB EXAM 1 Nitrate Reduction, Citrate Urea Hydrolysis, SIM 2/11+2/13 Starch Hydrolysis Casein Gelatin 2/18+2/20 NO LAB PRESIDENTS DAY Bacterial Transformation: pglo system Discussion of Unknowns Introduction to Bergey s Receive Mixed Culture- Isolate Unknowns Receive notebooks Discussion of Flow chart 2/25+2/27 Isolate + Identify Unknowns Identify Unknowns 3 /4+3/6 Investigating Eukaryotes Handwashing Lab Fungi, tardigrades and pond water Receive Unknown if not isolated Identify Unknowns
3/11+3/13 Membrane Filter Technique Bring Samples Isolate + Identify Unknowns Coagulase Antimicrobial Susceptibility Test Identify Unknowns 3/18+3/20 Identify Unknowns Lab Exam #2 Your textbook has much more detail about all of these stains and tests. I highly recommend that you read this material in your textbook also. Lecture Schedule Week Day Date Lecture Topic Reading 1 M 1/7 Introduction Chapter 1 1 W 1/9 Chemistry review Cell Anatomy Chapter 2 Chapter 4 2 M 1/14 Cell Anatomy Chapter 4 2 W 1/16 Cell Anatomy Chapter 4 3 M 1/21 NO CLASS MARTIN LUTHER KING JR DAY 3 W 1/23 EXAM #1 Chapters 1,2,4 4 M 1/28 Metabolism Chapter 5 4 W 1/30 Metabolism Chapter 5 5 M 2/4 Growth of Bacterial Cultures Genetics Chapter 6 Chapter 8 5 W 2/6 Genetics Chapter 8
6 M 2/11 EXAM #2 Chapters 5,6,8 6 W 2/13 Classification Prokaryotes Chapter 10 Chapter 11 7 M 2/18 NO CLASS PRESIDENTS DAY 7 W 2/20 Brief overview of eukaryotic microbes Chapter 12 8 M 2/25 Viruses Chapter 13 8 W 2/27 Viruses, viroids and prions Principles of Disease and Epidemiology 9 M 3/4 Principles of Disease and Epidemiology 9 W 3/6 Microbial Mechanisms of Pathogenicity Chapter 13 Chapter 14 Chapter 14 Chapter 15 10 M 3/11 EXAM #3 Chapters 10-14 10 W 3/13 Microbial Mechanisms of Pathogenicity Immune System Chapter 15 Chapter 17 11 M 3/18 Immune System Chapter 17 11 W 3/20 Immune System Control and Kill Microbes Chapter 17 Chapter 20 12 M 3/25 EXAM #4 Chapters 7,15,17,20