Course: Micr 450L/650L Infectious Disease Pathogenesis Lab Office: Van Es 124 Location: Van Es 107

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1 Micr 40L/0L Infectious Disease Pathogenesis Laboratory SPRING 14 Instructor Information: ourse Information: NAME: JANIE HAGGART ourse: Micr 40L/0L Infectious Disease Pathogenesis Lab Office: Van Es 124 Location: Van Es 107 Telephone: Day and Time: 12:00-1:0 PM T/TH Office hours: as arranged, Monday-Thursday Texts and Materials: Required: Infectious Disease Pathogenesis Laboratory and Lab Kit. A laboratory coat which must be kept in the lab until the completion of the term. Optional: A Photographic Atlas for the Microbiology Laboratory. M. J. Leboffe &. E. Pierce. ourse Description: The course will explore laboratory-based activities specifically designed for the microbiology major. Prerequisite: MIR 30L ourse Objectives: This course is specifically designed for the microbiology major. Through a series of laboratory-based activities, the successful student will be to accomplish the following: 1. To become familiar with individual pathogenic microorganisms by using proper aseptic technique, by selecting appropriate conventional and rapid identification biochemical media and tests, by using a dichotomous key, and by employing molecular tools 2. To use a variety of educational resources to help identify and understand pathogens 3. To understand and respect the importance of pathogenic microorganisms in human and animal health, as well as in agriculture 4. To understand how microorganisms exchange genetic information. To use microbiological information to enhance critical thinking skills to problem-solve. To work effectively in both individual and group settings 7. To develop the ability to think independently and discuss microbiological issues in an intellectual and informed manner 8. To develop skills which will help the student succeed professionally after attaining a degree in microbiology 9. To take personal responsibility for academic success ourse Policies: Safety: This course involves the use of pathogenic microorganisms. Standard operating procedures for iosafety Level 2 must be strictly adhered to, and for the safety of all students and instructors, any student not following these procedures will be dismissed from the course. Students are required to sign and turn in the safety sheet found in the laboratory manual before they are allowed to continue with the course. The signing of this sheet is a contract between the student and the instructor and allows the instructors to dismiss the student should any of the provisions be violated. Attendance: Per NDSU policy (Section 333), attendance in classes is expected. Only the course instructor can excuse a student from course responsibilities. (The term "course" includes class, laboratory, field trips, group exercises, or other credit-bearing activities.) If class attendance is a component of the course grade, the course instructor must clearly communicate this to the class in the syllabus. Non-attendance does not absolve students from course responsibilities and does not automatically remove courses from student academic records. The course instructor must inform students on the first day of class and in writing in the syllabus (1) of their policy regarding class absence and (2) policy, if any, for making up missed assignments. It is recognized that sometimes an assignment or examination is impossible to make-up. Although the course instructor should exercise a fair and consistent standard for resolving questions of missed assignments, the type, extent, manner, and time frame of the make-up assignments shall be at the discretion of the instructor.

2 Micr 40L Infectious Disease Pathogenesis Lab 14 Students are responsible for informing course instructors of absences. If absences are known (e.g., university sanctioned activity), students must inform course instructors with written notification as far in advance as possible (preferably a two-week notice). Where advance notification is not possible (e.g., illness, family emergency, etc.), students should contact their course instructor as soon as possible about the absence. When a student misses class for any reason, the student is expected to make arrangements with the course instructor to follow the course instructor's policy in making up any missed assignments, if permitted. Unexcused absences will result in the deduction of 1-10 points at the end of the semester. Academic Honesty: All students taking any course in the ollege of Agriculture, Food Systems, and Natural Resources are under the Honor System ( The Honor System is a system that is governed by the students and operates on the premise that most students are honest and work best when their honesty, and the honesty of others, is not in question. It functions to prevent cheating as well as penalize those who are dishonest. It is the responsibility of the students to report any violations of the honor pledge to the instructor, honor commission or the Dean of the ollege of Agriculture, Food Systems, and Natural Resources. All work in this course must be completed in a manner consistent with NDSU University Senate Policy, Section 33: ode of Academic Responsibility and duct ( All homework assignments must be original documents worked on independently unless directed otherwise by the instructor. itations are required to prevent plagiarism. Students with special requirements: Any students with disabilities or other special needs, who need special accommodations in this course are invited to share these concerns or requests with the instructor as soon as possible. The instructor may ask for verification and that, plus other assistance, can be requested from Disability Services in Wallman Wellness enter 0 ( ). Veterans and military personnel: Veterans or military personnel with special circumstances or who are activated are encouraged to notify the instructor as early as possible. Mission Statement for Department of Veterinary and Microbiological Sciences: VMS serves the local, national and international communities by teaching the concepts and applications of microbiology in a student-centered environment and by using our unique expertise to explore novel research in microbiology and disease pathogenesis. Our department values lifelong discovery, intellectual integrity, collegiality, and diversity. lackboard: All students are to be enrolled in lackboard. Online quizzes, assignments other pertinent information will be available at this site. It is your responsibility to check before each class period for pre-class assignments/quizzes. You will not be sent reminders. omputers: Throughout the semester there will be many times it will be necessary to use computers. It is the desire of the instructors to minimize the out-of-class time necessary to complete most activities. Therefore, the computer lab in Van Es has been reserved during this class period for the entire semester for your use. If you prefer, please feel free to bring a laptop with you. You will not be allowed to use your computer or cell phone when you are working with microorganisms. Graduate students: In addition to the above course objective, graduate students will be expected to acquire a higher level of proficiency and understanding of microbiological techniques. These expectations will be met by participation in an actual case study in cooperation with the N. D. State Veterinary Diagnostic Lab. A written report on the case study, done in consultation with the instructor, will be required. Also, an oral report on the case study will be presented to the class. These reports will be worth an additional 2 points for a total of 30 points

3 Micr 40L Infectious Disease Pathogenesis Lab 14 Assessment: Student assessment will be through online quizzes and assignments associated with a series of activities performed throughout the semester. All assignments will be due at the beginning of the class on the date stated in the syllabus. LATE ASSIGNMENTS WILL NOT E AEPTED UNLESS THERE IS A ASE OF SERIOUS ILLNESS OR PERSONAL RISIS. Documentation confirming the illness or personal crisis must be presented to the instructor before arrangements will be made. Assignment Possible Points My Points Due Date Experiment 4: Disinfectants 10 Thursday, Jan 23 Experiment 3: Koch s Postulates 10 Thursday, Jan 30 Scott Special 10 Thursday, Feb Activity 1: Exchange of Genetic Information 30 Thursday, Feb Activity 2: Epidemiology and ELISA 30 Tuesday, Feb 2 Activity 3: Gram Positive occi 30 Tuesday, March 11 Activity 4: Gram Negative acilli 30 Tuesday, April 1 Activity : Mixed Unknown Project 0 Tuesday, April 29 Activity : Lab Set up Thursday, May 1 Activity 7: ase Study Tuesday, May Online Quiz #1 Gram Positive occi Tuesday, March 2 Online Quiz #2 Gram Negative acilli and such Tuesday, April 1 Pre-lab quizzes/drop quizzes 2 Anytime Adopt an M/O 10 Assorted dates Total 32 40L Points/Grade 0L Points/Grade = A; = ; = ; = D; <194.9 = F = A; = ; = ; = D; <9 = F

4 Micr 40L Infectious Disease Pathogenesis Lab 14 WEEK PERIOD DATE / EXPT/ Jan 14 T Jan 1 TH / / ATIVITY Ex 1 Ex 2 Ex 1 Ex 4 Experiment heck in/pretest Review of Microbiological Techniques Rapid Method for Detection of Gram Reaction Koch s Postulates: preview Examination of prepared slides if necessary Review of Microbiological Techniques Koch s Postulates: 1 Disinfectants: 1 PAGE Jan 21 T Ex3 Ex 4 Koch s Postulates: 2 Disinfectants: Jan 23 TH Act 1:P1 Koch s Postulates: 3 Activity 1: Exchange of Genetic Information: Transduction: 1 Activity 1: Exchange of Genetic Information: Transformation: Jan 28T Act 1:P1 Koch s Postulates: 4 Activity 1: Exchange of Genetic Information: Transduction: 2 Activity 1: Exchange of Genetic Information: Transformation: Jan 30 TH Activity 1: Exchange of Genetic Information: Transformation: Feb 4 T TA Activity 1: Exchange of Genetic Information: Transformation: 4 Scott Special 7 8 Feb TH Activity 1: Exchange of Genetic Information: acterial jugation: 1 9 Feb 11 T Activity 1: Exchange of Genetic Information: acterial jugation: 2 Activity 2: Epidemiology/ELISA: 1 10 Feb 13 TH Activity 1: Exchange of Genetic Information: acterial jugation: 3 Activity 2: Epidemiology/ELISA: 2 11 Feb 18 T EM Tour 12 Feb TH Activity 2: Epidemiology/ELISA: 3 Activity 3: Gram Positive occi: Feb 2 T Activity 3: Gram Positive occi: 2 14 Feb 27 TH Activity 3: Gram Positive occi: Mar 4 T Activity 3: Gram Positive occi: 4 1 Mar TH Activity 3: Gram Positive occi: 9 Mar 11 T Activity 3: Gram Positive occi: 18 Mar 13 TH Out-of-class activity

5 Micr 40L Infectious Disease Pathogenesis Lab 14 Mar -21 NO LASS SPRING REAK Mar 2 T Activity 4: Gram Negative acilli: 1 Mar 27 TH Activity 4: Gram Negative acilli: Apr 1 T Activity 4: Gram Negative acilli: 3 22 Apr 3 TH Activity 4: Gram Negative acilli: Apr 8 T Activity 4: Gram Negative acilli: 24 Apr 10 TH Activity 4: Gram Negative acilli: Apr 1 T Apr 1 W Apr TH Activity : Mixed Unknown Project : 1 Please note: you will need to come in on Wednesday Activity : Mixed Unknown Project : Apr 22 T Act Activity : Mixed Unknown Project : 3 Activity : Lab set up: Apr 24TH Act Activity : Mixed Unknown Project : 4 Activity : Lab set up: Apr 29 T Act Activity : Lab set up: May 1 TH Act Activity : Lab set up: 4 Presentation May T May 8 TH Act 7 Activity 7: ase Study Make-up day (if needed)