NATURAL SCIENCE II: HUMAN GENETICS
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1 NATURAL SCIENCE II: HUMAN GENETICS V Spring 2005 Tuesdays and Thursdays 9:30 A.m. 10:45 A.m. Silver 207 Dr. Burt Goldberg Associate Professor of Biochemistry Chemistry Department Phone: Office Hour: By appointment Room 1152 Brown 1
2 1. Course Description We are currently witnessing a revolution in human genetics, where the ability to scrutinize and manipulate DNA has allowed scientists to gain unprecedented insights into the role of heredity. This course explores the foundations and frontiers of modern human genetics, with an emphasis on understanding the latest discoveries in this rapidly changing field of research. The course begins with an overview of cell structure and cellular processes. We then examine the principles of heredity, where simple Mendelian inheritance is contrasted with the effect of multiple genes and the environment that influence complex physical or behavioral traits. Descending to the molecular level, we investigate how genetic information is encoded in DNA and how mutations affect gene function. These molecular foundations are used to explore the science and social impact of modern genetic technology, including topics such as cloning, genetic testing, and the new map of the human genome. The course concludes with an investigation of how genes vary in populations and how geneticists are contributing to our understanding of human evolution and diversity. The laboratory projects throughout the semester are designed to introduce students to techniques currently used by scientists, ranging from diagnosing inherited traits using family pedigrees to hands-on explorations of modern methods of genetic testing. 2. Course Objectives To acquire foundational knowledge of the biological mechanisms of inheritance. To develop skills in problem solving and interpreting scientific information. To engage in various methods of scientific investigation in the laboratory. To critically evaluate popular media reports on the latest developments in genetics research. To address the complex ethical, social and legal consequences of genetic information. 3. Course Texts and Readings Required Texts Ricki Lewis, Human Genetics: Concepts and Applications, 6 th Edition (McGraw Hill, 2004). This text comes bundled with a free CD-ROM. Human Genetics Laboratory Manual. Recommended Texts James D. Watson, The Double Helix: A Personal Account of the Discovery of the Structure of DNA (Touchstone Books, 2001). News Articles Articles about recent discoveries in genetics from The New York Times or other sources will be assigned throughout the semester as part of the required readings. 2
3 4. Lecture, Reading, and Laboratory Schedule Readings from Ricki Lewis, Human Genetics, are indicated by the letter L followed by the chapter and page numbers. The recommended text by James D. Watson, The Double Helix, is best read throughout the first part of the course in preparation for lectures 11 and 12. Date Lecture Topic Reading Laboratory Project 1 Jan Introduction and Course L Ch. 1, pp No laboratory T 18 Overview CELLS AND CHROMOSOMES 2 Th Jan Structure and Function of Cells L Ch. 2, pp T Jan 25 W Jan 26 4 Th Jan 27 The Cell Cycle and Mitosis L Ch. 2, pp Lab 1: Microscopy of Cells Meiosis & Cytogenetics L Ch. 3 pp L Ch. 12, pp T Feb 1 Science & Ethics of Cloning L Ch. 2, pp L Ch. 3, pp W Feb 2 Lab 1: Microscopy of Cells Lab 2: Mitosis Lab 2: Mitosis PRINCIPLES OF INHERITANCE Mendelian Inheritance L Ch. 4, pp Th Feb 3 7 T Feb 8 Sex-Linked Traits L Ch. 6, pp W Feb 9 8 Th Feb Extensions to Mendel s Laws L Ch. 5, pp T Feb L Ch. 7, pp W Feb Th Feb T Feb 22 Multifactorial Traits & Statistics Proposal for Media Report Due Genetics of Multifactorial Traits L Ch. 7, pp Genes & Behavior L Ch. 8, pp Lab 3: Meiosis Lab 3: Meiosis Lab 4: Human Genetic Traits Lab 4:Human Genetic Traits Review for Midterm 1 3
4 W Feb 23 Th Feb 24 Midterm Exam 1 Review for Midterm 1 DNA & GENETIC INFORMATION 12 T Mar 1 The DNA Double Helix Part 1 W Mar 2 13 Th Mar 3 The DNA Double Helix Part 2 14 T Mar 8 Expression of Genetic Information L Ch. 9, pp Watson, Double Helix L Ch 9., pp Watson, Double Helix L Ch. 10, pp Lab 5: Isolation of DNA Lab 5: Isolation of DNA Lab 6: : Sickle Cell Anemia W Mar 9 15 Th Mar 10 Gene Mutation L Ch. 11, pp Lab 6: : Sickle Cell Anemia SPRING RECESS (March 15 19) 16 T Mar 22 W Mar Th Mar 24 GENETIC TECHNOLOGY Recombinant DNA Technology L Ch. 18, p Human Genome Project & Genomics L Ch. 22, pp Reproductive Technologies L Ch. 21, pp T Mar 29 W Mar Th Mar Medical Genetics T Apr 5 Gene Therapy & Genetic Counseling TBA L Ch. 19, pp Lab 7:Blood Type and Population Genetics Lab 7:Blood Type and Population Genetics Student Presentations Student Presentations Review for Midterm 2 4
5 W Apr 6 Review for Midterm 2 Th Apr Midterm Exam T Apr 12 W Apr Th Apr T Apr 19 W Apr Th Apr T Apr 26 W Apr Th Apr T May 3 GENES AND POPULATIONS Blood Types in Human Populations Principles of Population Genetics L Ch. 5, pp L Ch. 13, pp DNA Fingerprinting L Ch. 13, pp Genes & Evolution Completed Media Report Due Drug Resistant Tuberculosis & Influenza Virus Conclusion: Our Genetic Future REVIEW for FINAL L Ch. 14, pp Handout Lab 8:DNA Fingerprinting Lab 8:DNA Fingerprinting T.B.A. T.B.A. Review & Evaluations Review & Evaluations 5. Grade Allocation Midterm Exam 1 25 % Midterm Exam 2 25 % Final Exam 30 % Laboratory & 25 % Homework Grading; 100 to 95 = A, 94 to 90 = A-, 89 to 85 = B+, 84 to 80 = B, 79 to 75 = C+, 74 to 70 = C, 69 to 65 = D. In-class assignments will be given regularly in lectures and some of these will be collected and reviewed. There will be no formal grade component for these assignments, but they will be used in deciding cases of borderline grades at the end of the semester. 5
6 6. Coursework and Policies If you miss lecture or laboratory, you are still responsible for work assigned and work that was to be collected! Exams The exams will contain questions covering the lectures, readings, and laboratory projects. Study questions for the lecture topics will be distributed regularly during the semester. The final exam will be cumulative and will cover topics from throughout the course. Homework assignments provide practice with some types of questions that will appear on the exams. If you will miss one midterm exam because of illness, you must contact Professor Goldberg by e- mail before the start of the exam and provide a doctor's note explaining your absence. No makeup exams will be given for the course. MAKEUP EXAMS ARE NOT A BIRTH RIGHT! Instead, the final exam will count as 55% of your course total. Since the final is cumulative and the most difficult exam of the course, this option is not advisable unless extreme circumstances prevail. If you miss two midterm exams you will be required to withdraw from the course. A make-up will be given for the final exam only under VERY exceptional circumstances that must be discussed with Professor Goldberg prior to the FINAL EXAM. In this case a grade of Incomplete will be given for the course and the make-up will be scheduled for the Fall 2005 semester. No alternative date for the final exam will be offered at the end of the spring semester so avoid making travel plans to leave NYU before the date of the final exam. THIS IS AN ABSOLUTE! Homework The homework assignment will contain questions that review the course material and/or questions that relate to the laboratory. Certain questions on the homework assignments may require you to access information on relevant web sites. Each homework assignment will contain several questions, of which one will be graded. Assignments are worth 5 points, with up to 3 points for the graded question and 2 points for completing all the questions. All homework must be submitted on time for full credit. Any late assignments will be penalized 1 point per day (excluding weekends). If you miss a lecture or laboratory session due to a documented absence you are still required to complete the homework assignment. Contact your laboratory instructor to arrange a suitable deadline for submitting the work. Media Assignment One important component of scientific literacy is the ability to insightfully read an article about science in the popular press. This involves understanding the scientific vocabulary, the methods used in the investigation, what type of data was generated, and the validity of the conclusions. The Human Genetics course seeks to foster this skill through the semester and it will also be used as a component of the assessment. Some of the best science reporting is provided by The New York Times, both in the weekly Science Times section on Tuesdays and in daily coverage throughout the week. You will have the opportunity to work collaboratively on selecting and evaluating a NY Times article that appeals to your interests. The project will be developed in three stages: a proposal (due on February 15), a 6
7 presentation in the laboratory session (March 29 & 30), and a final report (April 21). Further details about this project will be provided early in the semester. 7. Laboratory Sessions The laboratory sessions will be held in Silver 201. There will be no laboratory session during the first week of classes and the sessions will begin on Tuesday, January 25. The laboratories will be taught by three experienced instructors. Lab Sec. Day and Time Lab Instructor 1 Tuesday 3:00-4:40 SILAS HOXIE sjh276@nyu.edu 2 Tuesday 5:00-6:40 SILAS HOXIE. 3 Wednesday 9:00-10:40 MIKE LEVY mdl275@nyu.edu 4 Wednesday 11:00-12:40 MIKE LEVY 5 Wednesday 1:00-2:40 STEVE KOUTSOYIANNIS sk1181@nyu.edu 6 Wednesday 3:00-4:40 STEVE KOUTSOYIANNIS Laboratory Policies Each weekly experiment is worth 50 points: Attendance Quiz Lab Assignment 10 points 10 points 30 points You must be registered in a laboratory section in order to receive credit for the course. The sections have a capacity of 20 students, which is determined by the availability and logistics of laboratory equipment. If you are not appropriately registered for a laboratory section by project #2 you will be required to drop the course. You are expected to arrive punctually for the beginning of the lab session. Arriving more than 10 minutes late will result in a loss of attendance credit for the session. Questions for the laboratory quiz will be based on the description of the experiment in the laboratory manual and may also include pertinent material from the lectures and readings. Arriving more than 10 minutes late for the lab will exclude you from taking the quiz. The laboratory assignment must be completed and submitted during the laboratory period by working collaboratively with your laboratory partners. Some laboratories may have a take-home component in addition to the in-lab exercises. 7
8 You will be at a disadvantage in the course if you miss any of the lab sessions. If you cannot attend a lab session because of illness, notify your lab instructor before the start of the laboratory session and provide doctor s note within one week. If you will miss a lab session due to special circumstances, including observation of a religious holiday, notify your lab instructor in advance. Because of the logistics of using the laboratory room, it is not possible to attend another laboratory section other than your own or to perform make-up experiments. Missing more than three laboratory sessions for any reason will result in receiving a score of zero for the entire laboratory portion of the course. THIS IS NOT A FORGIVABLE SIN! 8
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