General Genetics Biology 184 Fall 2005
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1 General Genetics Biology 184 Fall 2005 Instructor: Dr. Tom Peavy Office: SQU 406 Phone: Office hours: Tues & Thurs 1:00-2:30pm (and by appointment) I. Class times Lecture: Lab Sections: Section 5, T/R 3:00-3:50 PM (HUM-202) Section 6, T/R 9:00-10:15 AM (HUM-220) Section 7, T/R 10:30-11:45 AM (HUM-220) II. Course Prerequisites: Microbiology (BIO 139 or equivalent; Chemistry majors are exempt) Organic Chemistry (CHEM 20 or equivalent) III. Books Lecture Textbook : Genetics Analysis & Principles (2005) Second edition By Robert J. Brooker Online supplements with the textbook ( Laboratory Paperback book: Beyond Therapy: Biotechnology and the Pursuit of Happiness, a Repor t by the President s Council on Bioethics (2003); Bio 184 Laboratory Manual (Ballard and Holland, 2003) IV. Course Objectives: The overall course objective is to increase student knowledge, awareness and appreciation of classical and molecular genetics with emphasis on the formation, transmission, function and organization of the genetic material. Learning Outcomes: Specifically you will be able to Understand a historical perspective of genetics, identifying breakthroughs of discovery, and prominent scientists who were involved in these breakthroughs. 2. Use problem-solving skills to predict genetic outcomes. 3. Describe basic inheritance patterns and the chromosomal basis of heredity. 4. Explain mutation as a source of genetic variability. 5. Understand the role of sex chromosomes in sex determination, sexual dimorphism and chromosomal inactivation. 6. Understand how cells reproduce through DNA and nucleic acids. 7. Describe how DNA transcribes into RNA that ultimately translates into protein. 8. Articulate some of the major issues related to modern biotechnology and genetic manipulation. 9. Develop skills in analysis, problem solving, communication and ethical perspectives as it applies to genetics.
2 V. Course Structure: Lectures will provide the students with the conceptual framework of genetics and develop problemsolving skills. Particular emphasis will be given to understanding the scientific method and scienceas-way-of-knowing through hypothesis-driven research. Lecture materials will be available one-day prior to lecture on the instructors course website: ( Laboratory will be a combination of learn-by-doing exercises (illustrating central themes of genetics), written laboratory reports (developing analytical, evaluation, and presentation skills), and bioethical discussions and presentations (enhancing student appreciation for the complex issues surrounding genetic technologies). VI. Evaluation: The combined lecture portion of the course will be worth 70% of the student grade (360 pts) and laboratory will be worth 30% (150 pts). Lecture A) Homework 20 pts each) 60 pts B) Examinations 100 pts each) 300 pts Total Lecture points 360 pts Laboratory C. Lab Participation* 15 pts D. Lab Reports 10 pts)* 60 pts E. Group Discussion/Presentation 30 pts F. Lab Practical Exam* 45 pts Total Laboratory Points 150 pts *Service-learning option with Project R.I.D.E. (see service-learning handout for details) ½ of lab participation points are determined by participation in Project R.I.D.E. EXEMPT from Lab Practical Exam (service-learning project substitutes) Final grades will be determined on a percentage basis of the total points possible ( = 510) according to the following scale: % A % C % A % C % B % D % B % D % B % D % C+ < 60 % F
3 A. Homework assignments: Students are responsible for completing homework assignments on time to receive full credit. Late assignments will be docked four points for every day late (including weekend days). B. Exams: There will be three exams worth 100 points each given during class (see syllabus for dates). Each exam will cover the material from lectures and homework. The format will be multiple choice, short answer, and problem-solving questions. A review will be given during the class prior to each exam. Students are expected to come to the review prepared to ask questions. You will have the entire 50 minutes to take the exam. The only resource you may use on any exam or quiz will be your pencil (bring two sharpened pencils with erasers). You can not use calculators, PDA's or cell phones unless told otherwise. The third and final exam will not be cumulative but rather cover the material since the previous exam. A make-up exam will be given to a student only if the circumstances surrounding their absence was beyond their control, as deemed by me. In this case, I will require written documentation to verify the circumstance within one week of the missed exam. C. Lab Participation In order to participate, a student must attend the labs. Students that miss more than three laboratory periods will receive a U in the course. Points for lab participation will be divided between attendance, effort in lab, and participation in discussions. D. Lab Reports Written lab reports will be due one week after the completion of the laboratory exercise. The specific information regarding the format and content for each lab will be given to students in lab when performing the experiment. Late reports will be docked 2 pts/day (weekends included) E. Lab Group Discussion Four laboratory sessions are reserved for group presentation and discussion of four major topics of biotechnology and bioethics based on the report Beyond Therapy: Biotechnology and the Pursuit of Happiness from the President s Council on Bioethics. Groups of four students will provide a 30 minute PowerPoint presentation summarizing the issues surrounding the major topic and lead a 15 minute discussion. Each student will be graded separately on their participation and presentation. Additional information will be provided on the particulars of each group presentation. F. Lab Pract cal Exam i An examination focusing on the laboratory skills, operation of equipment, and theoretical concepts addressed in lab will occur during the last week of laboratory (Thurs, December 8). A review session will be provided during the lab prior to the examination.
4 VII. Course policies Cheating and plagiarism are not allowed. Cheating and plagiarism are very serious offences and will be prosecuted accordingly. The Department Chair and Dean of Students will be notified of the event and the student may be dismissed from the course with an F. Other possible lesser actions may be the loss of all points for the assignment/quizz/exam and/or reduction of the students final grade. All quizzes and exams are closed book and notes. Plagiarism is defined by the University as the use of distinctive ideas or works belonging to another person without providing adequate acknowledgement of that person's contribution. Students should review the University policy on plagiarism provided on the web ( ). VIII. Add/Drop Policy The C.S.U.S Class Schedule details the University s add/drop policy. Students should evaluate whether they have the time to devote to the class before adding the class. In order to drop the course after the Census Date, students are required to provide documentation to verify their extenuating circumstances. Forms are available in the Biological Sciences Department office. IX. Disabilities Assistance will be provided to all individuals with a disability that affects their ability to succeed in the course. Students with disabilities should contact me as soon as possible (preferably in the first week) to arrange for reasonable accommodations. X. Common Sense Rules A. All pagers and phones should be turned off (or set to vibrate) during class time. I reserve the option of confiscating the phone or pager from a student until the end of class if it becomes a problem. B. We should be respectful to each other and listen when anyone else is asking questions or when I am lecturing. Please do not talk or interrupt others. C. If a student arrives late or needs to leave early, take a seat near the door to avoid disrupting the class. D. Students should only be concentrating on Bio I84 during the lecture and therefore should not have other reading material or assignments on their desk during class time.
5 Bio 184 Spring 2005 * Lecture Schedule Date Topic Reading (Brooker) Homework due date 30-Aug Introduction to Course & Service Learning Chapter 1 1-Sep Mendelian Inheritance & Principles Chapter 2 6-Sep Punnett Squares/ Probabilities Chapter 2 8-Sep Human Genetics/Pedigrees Chapter 2 13-Sep Transmission of DNA (Mitosis & Meiosis) Chapter 3 15-Sep Meiosis Cont'd and Chromosomal Theory Chapter 3 20-Sep Sex-linked Transmission Chapter 3 22-Sep Linkage & Genetic Mapping in Eukaryotes Chapter 5 27-Sep Extensions of Mendelian Inheritance Chapter 4 29-Sep Extensions of Mendelian Inheritance (cont'd) Chapter 4 HW #1 4-Oct Review for Exam 1 6-Oct Exam 1 11-Oct Chromosome Structure and Recombination Chapter 8 13-Oct Chromosomal Number Variations Chapter 8 18-Oct Molecular Structure of DNA and RNA Chapter 9 20-Oct Chromosomal Organization & Molecular Structure Chapter Oct DNA Replication Chapter Oct DNA Replication (cont'd) Chapter 11 1-Nov Gene Transcription Chapter 12 HW #2 3-Nov Review for Exam 2 8-Nov Exam 2 10-Nov Translation of mrna Chapter Nov Gene Regulation in Prokaryotes Chapter Nov Gene Regulation in Prokaryotes cont'd Chapter Nov Gene Regulation in Eukaryotes Chapter Nov Thanksgiving Holiday 29-Nov Gene Mutation Chapter 16 1-Dec Quantitative Genetics Chapter 24 6-Dec Evolutionary Genetics Chapter 26 HW #3 8-Dec Review for Final exam 13-Dec FINAL EXAM (3:00-5:00PM) * Subject to change
6 * LABORATORY SCHEDULE WEEK LAB EXERCISE READING IN LAB MANUAL 8/30 9/1 9/6 9/8 Course orientation Lab safety; Student introductions Experiment 1, Day One: Introduction to Drosophila and linkage mapping Experiment 1, Day Two: Set up cross for genetic mapping ii-iv /13 9/15 9/20 9/22 9/27 9/29 10/4 10/6 10/11 10/13 10/18 10/20 10/25 10/27 11/1 11/3 11/8 11/10 Experiment 1, Day Three: Drosophila life cycle Experiment 1, Day Four: Probabilities and pedigrees Experiment 1, Day Five: Chi-Square analysis Experiment 1, Day Six: Count and score flies Experiment 1, Day Seven: Finish counting and scoring flies; map gene loci Presentation 1: Better Children Experiment 2, Day One: Introduction to light microscope; LAB REPORT 1 DUE Experiment 2, Day Two: Human karyotyping Experiment 2, Day Three: Abnormal human karyotypes Presentation 2: Superior Performance Experiment 3, Day One: Introduction to molecular genetics equipment; LAB REPORT 2 DUE Experiment 3, Day Two: Creating a recombinant DNA molecule Experiment 3, Day Three: Agarose gel electrophoresis Presentation 3: Ageless Bodies Experiment 4, Day One: Introduction to human DNA identification; LAB REPORT 3 DUE Experiment 4, Day Two: Isolation of cheek cell DNA Experiment 4, Day Three: Set up PCR reactions Experiment 4, Day Four: Analysis of CGE run and profiling results /15 11/17 Presentation 4: Happy Souls Experiment 5, Day One: Enzyme induction; LAB REPORT 4 DUE /22 11/24 11/29 12/1 Experiment 5, Day Two: Enzyme assay Thanksgiving Holiday Experiment 6, Day One: Introduction to heritability and regression; Experiment 6, Day Two: Regression analysis of height data; (Meet at 2003 Solano Hall); LAB REPORT 5 DUE /6 12/8 * Subject to change Review for lab practical exam; LAB PRACTICAL EXAM; LAB REPORT 6 DUE 1-80
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